| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Digiovanni, Amaya M (15)
|
ADDACC |
53.35 |
55.60 |
2.25 |
| 2 |
Orcutt, Nicole E (17)
|
ADGBY |
54.78 |
55.87 |
1.09 |
| 3 |
Gilbert, Portia M (16)
|
ADGLEN |
58.68 |
58.21 |
-0.47 |
| 4 |
Lashway, Elizabeth G (15)
|
ADGUY |
57.05 |
58.48 |
1.43 |
| 5 |
SantaBarbara, Marianna (15)
|
ADDACC |
55.54 |
59.41 |
3.87 |
| 6 |
Wolfstich, Ella G (16)
|
ADGLFY |
56.15 |
59.95 |
3.80 |
| 7 |
Koeferl, Alexandra M (17)
|
ADGLEN |
56.24 |
1:00.10 |
3.86 |
| 8 |
Gillan, Elaine M (15)
|
ADGBY |
1:02.26 |
1:00.84 |
-1.42 |
| 9 |
Buzas, Megan R (15)
|
ADDACC |
57.81 |
1:01.74 |
3.93 |
| 10 |
Hawthorne, Julia E (17)
|
ADSARY |
56.52 |
1:01.96 |
5.44 |
| 11 |
Barnhart, Breityn O (16)
|
ADDACC |
59.47 |
1:02.15 |
2.68 |
| 12 |
Maier, Madison K (16)
|
ADGLFY |
57.36 |
1:02.56 |
5.20 |
| 13 |
Frenyea, Sophia J (17)
|
ADGLOY |
1:03.50 |
1:03.03 |
-0.47 |
| 14 |
Rutkowski, Janna K (16)
|
ADKING |
1:00.13 |
1:05.49 |
5.36 |
| 15 |
Norris, Julia M (16)
|
ADGLOY |
1:07.12 |
1:07.26 |
0.14 |
| 16 |
Rizzo, Abigail E (16)
|
ADGLOY |
1:07.40 |
1:08.60 |
1.20 |